Biowell Technology Inc. is the exclusive agent and distributor in Taiwan for the PSQ96 Pyrosequencer from Pyrosequencing AB, Sweden. For inquiries within Taiwan about purchasing this equipment, please contact Biowell.

PSQ96 Pyrosequencer Instrument Characteristics
Unique enzymatic system without the need for gel, staining, or fluorescence label.
Employing solid phase magnetic beads for easy and fast sample preparation.
96-well sample tray allows 96 post-PCR samples to be analyzed in 10 minutes.
Online sequence detection, no waiting period.
Single nucleotide polymorphism (SNP) accuracy higher than 99%.
Suitable for short and medium length DNA sequencing.
Equipped with Windows NT for easy operation.
Best designed for sequencing of large numbers of short length DNA.

PSQ96 Pyrosequencer Application Field
Gene mutation screening:
  can screen gene mutation caused by SNP in a known sequence (such as cervical cancer).
Obesity gene screening:
  can screen obesity caused by gene mutation.
Population SNP haploid gene frequency estimation:
  can greatly reduce the cost of population studies.
Hypertension factor screening:
  can screen patients with hypertention factor ACE (angiotension converting enzyme).

Comparison of pyrosequencing system with other SNP systems
System
Method
Advantage/disadvantage

Pyrosystem
CR, ATP sulfurylase, luciferase, apyrase
Analysis by synthesis
Instant sequencing
Precision results
Need to know wild type sequence
dHPLC
PCR, dHPLC, sequencing
Can check larger DNA fragments
No need to know wild type sequence
Need further sequencing to locate sites
TAQMAN
PCR, fluorescence
Can check larger DNA fragments
No need to know wild type sequence
Need further sequencing to locate sites
Invader
Cleavase, hybridization, fluorescence
Can check larger DNA fragments
No need to know wild type sequence
Need further sequencing to locate sites
Microarray
DNA probes, hybridization, PCR
Can deal with lots of samples
Need to know all sequences
DNA pools
Quantitative PCR, cyber green
Can check larger DNA fragments
No need to know wild type sequence
Need further sequencing to locate sites
Sequencing
cloning, sequencing
Can find exact location of SNP
Cost and time consuming